Sequence 717 (LPA2)

Sequence LPA2
Target	LPAR2 ( Homo sapiens )
Description	Endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 4 Ensembl: ENSG00000064547 UniGene: Hs.122575 EntrezGene: 9170 Ensembl Chr19: 19595477 - 19600017 Strand: -1 GO terms: 0001584 0001619 0004872 0004930 0004949 0004977 0005515 0005886 0005887 0007165 0007186 0007202 0007204 0008289 0016020 0016021 0030274
Design	siRNA
Chemistry	RNA
Sequence	siRNA sense (19b) GGTCAATGCTGCTGTGTAC / siRNA antisense (19b) GTACACAGCAGCATTGACC
Application	gene silencing
Name	LPA2

References

G protein-coupled lysophosphatidic acid receptors stimulate proliferation of colon cancer cells through the {beta}-catenin pathway.Yang M, Zhong WW, Srivastava N, Slavin A, Yang J, Hoey T, An S.Proc Natl Acad Sci U S A. 2005 Apr 26;102(17) :6027-32. Epub 2005 Apr 18.

Intrathecal Injections in Children With Spinal Muscular Atrophy: Nusinersen Clinical Trial Experience. Hache M, Swoboda KJ, Sethna N, Farrow-Gillespie A, Khandji A, Xia S, Bishop KM. J Child Neurol. 2016 Jun;31(7):899-906. PubMed:26823478

Background

Description. The lysophospholipids, lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P; see 601974), are important extracellular signaling molecules. These lipid mediators are pleiotropic; among the most common cellular responses are mitogenesis, cell survival (antiapoptosis), inhibition of adenylyl cyclase, and calcium mobilization. Physiologic events associated with these mediators include platelet aggregation, vasopressor activity, wound healing, immune modulation, and angiogenesis. Many of the actions of LPA and S1P are mediated through a set of G protein-coupled receptors, including EDG4 (summary by Chun et al., 2002). Gene Function. Using the aequorin luminescence method to reduce nonspecific signals, An et al. (1998) determined that LPA induced increased calcium mobilization in cells expressing EDG2 or EDG4, probably through inositol triphosphate generated by phospholipase C activation. EDG4-mediated calcium mobilization utilizes both Gi (see GNAI1; 139310) and Gq (see GNAQ; 600998) proteins, whereas EDG2 utilizes pertussis toxin-sensitive Gi proteins only.

By RT-PCR and Western blot analysis, Goetzl et al. (2000) showed that CD4+ T cells and B cells but not CD8+ T cells express EDG4. Stimulation of CD4+ but not CD8+ T cells in the presence of LPA or monoclonal anti-N-terminal EDG4 suppressed interleukin-2 (IL2; 147680) secretion.